TABLE 2.
Time of bleeding (day p.i.) | Detection of CSFVa in blood from the indicated animal
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
V-H297K\
|
V-C171S
|
wt
|
||||||||
1186 | 1187 | 1188 | 1190 | 1191 | 1192 | 1193 | 1196 | 1197 | 1198 | |
−1 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
2 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
5 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ |
8 | +/+ | +/+ | −/− | +/+ | −/− | +/+ | +/− | +/+ | +/+ | |
12 | +/− | +/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | |
15 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | −/− | |
21 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
Viruses used for infection: V-H297K, RNase-negative virus (group 1); V-C171S, dimerization-negative mutant (group 2); wt, CSFV Alfort/Tübingen (group 3). One animal in group 3 had to be euthanized on day 5 p.i. +/+, virus positive in both wells of culture plate; +/−, only one well positive; −/−, both wells negative.