FIG. 4.

Colocalization of CKB with HCV NS4A. (A) Indirect immunofluorescence analysis. The primary antibodies used were anti-CKB goat PAb (red) and anti-NS4A MAb (green). Merged images of red and green signals are shown. High-magnification panels are enlarged images of white squares in the merge panels. (B) Immunoelectron microscopic localization of CKB and NS4A. SGR-N cells were double-immunolabeled for CKB (12-nm gold particles; white arrows) and for NS4A (18-nm gold particle; gray arrows). Mi, mitochondria. Bars, 200 nm.