TABLE 1.
Selected proteins that reproducibly increased in the DRM fraction of SGR-N cellsa
Avg ratio | P (Student t test) | Coverage (%) | Protein name | Molecular function | GI no. |
---|---|---|---|---|---|
5.56 | 0.04 | 27 | GRP94 | Protein folding | 15010550 |
4.99 | 0.07 | 47 | Hsp60 | Protein folding | 6996447 |
3.73 | 0.07 | 6 | tRNA guanine transglycosylase | Metabolism | 30583205 |
3.56 | 0.06 | 23 | KIAA0088 | Unknown | 577295 |
3.32 | 0.07 | 4 | Thioredoxin-related protein | Unknown | 20067392 |
3.32 | 0.13 | 12 | Tat binding protein 1 (TBP-1) | Cellular processes | 20532406 |
3.06 | 0.14 | 22 | Aldehyde dehydrogenase 1 | Metabolism | 2183299 |
3.06 | 0.14 | 14 | Chaperonin TRiC/CCT, subunit 2 | Protein folding | 54696794 |
2.96 | 0.04 | 14 | Heat shock 70-kDa protein 4 (HSPA4) | Protein folding | 6226869 |
2.96 | 0.04 | 29 | GRP58 | Metabolism/protein folding | 2245365 |
2.94 | 0.01 | 37 | Mutant β-actin | Cytoskeleton organization | 28336 |
2.65 | 0.17 | 33 | Glutathione S-transferase (GST) | Catalytic activity | 2204207 |
2.53 | 0.04 | 37 | Keratin 19 | Cytoskeleton organization | 6729681 |
2.46 | 0.08 | 6 | Heterogeneous nuclear ribonucleoprotein K | Nucleic acid modification | 460789 |
2.45 | 0.001 | 13 | HMG-coenzyme A synthase | Metabolism | 30009 |
2.4 | 0.02 | 31 | CKB | Energy pathway/metabolism | 180570 |
2.4 | 0.02 | 11 | Cathepsin D | Cellular processes | 30582659 |
2.4 | 0.02 | 11 | C8orf2 | Unknown | 37181322 |
2.36 | 0.1 | 38 | Tropomyosin 4-anaplastic lymphoma kinase fusion protein | Cytoskeleton organization | 14010354 |
2.36 | 0.1 | 6 | Calreticulin | Protein folding | 30583735 |
2.33 | 0.01 | 29 | Quinolinate phosphoribosyltransferase | Metabolism | 30583301 |
2.29 | 0.04 | 25 | Protein disulfide isomerase-related protein 5 | Protein folding | 1710248 |
2.29 | 0.04 | 16 | Tat binding protein 7 (TBP-7) | Cellular processes | 263099 |
2.05 | 0.11 | 24 | Calumenin | Metabolism | 2809324 |
2.05 | 0.12 | 10 | TRiC/CCT, subunit 5 | Protein folding | 24307939 |
2.03 | 0.07 | 20 | Hsp90 beta | Protein folding | 34304590 |
2.01 | 0.07 | 10 | TRiC/CCT, subunit 1 | Protein folding | 36796 |
The spectra obtained by tandem mass spectrometry were collected using data-dependent mode, and the results were subjected to database (NCBInr) search by Mascot server software (Matrix Science, London, United Kingdom) for peptide assignment. Coverage, the ratio of the portion of protein sequence covered by matched peptides to the whole protein sequence. GI no., GenInfo identifier number.