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AL2, AL21-114, and L2 cause overexpression of methylated, TGS-silenced loci. Arabidopsis plants containing the indicated transgenes under the control of a dex-inducible promoter were treated with dex (+) or water (−), and expression from the endogenous silenced loci was evaluated by sqRT-PCR. Actin served as a control. The data are representative of results obtained in at least two experiments using two independent lines for each transgene.
