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. 2009 Mar 11;83(10):4757–4765. doi: 10.1128/JVI.01927-08

FIG. 3.

FIG. 3.

Isolation of primary enveloped virions from infected nuclear membranes. (A) Western blot of SC16-infected TCE, INT, NMP, and pnHSV-1. Fractions were probed for the viral capsid protein VP5 and the nuclear membrane marker and endoplasmic reticulum marker calnexin. (B) Western blot of TCE, INT, NMP, pnHSV-1, and two variations of the pnHSV-1: (i) pnHSV plus proteinase K without centrifugation (−spin) and (ii) pnHSV without proteinase K and with centrifugation (+spin). Blots were probed for VP5, calnexin, and LAP-2, a nuclear membrane marker, as indicated at left. (C) TCE, pnHSV, and xHSV-1 were subjected to Western blotting. The Western blots were probed for viral capsid protein VP5 and for pUL31 and pUL34, markers of primary enveloped virions.