FIG. 2.

Enhanced acetylation of histones H3 and H4 of Myc chromatin in E1A-expressing cells. Chromatin prepared for ChIP assays as described in Materials and Methods was immunoprecipitated using anti-acetyl H3 (α-Ac-H3) and anti-acetyl H4 (α-Ac-H4) antibodies (catalog no. 06-599 and 06-866, respectively; Upstate). The immunoprecipitated DNA was quantified using real-time PCR with the primer pairs shown in Fig. 1A. The relative increase in real-time PCR values over those for the control samples (cells infected with Adb-gal) is shown. Each value shown is the average for two independent experiments with mean ± standard deviation). The commercial antibodies used in these studies were raised using acetylated peptides. The peptides included amino acids corresponding to 1 to 20 of H3, in which lysines 9 and 14 are acetylated, and 2 to 19 of H4, in which lysine 5, 8, 12, and 16 are acetylated.