FIG. 6.

E1A interferes in complex formation between p300, YY1, and HDAC3 in vitro. GST fusion proteins containing YY1, HDAC3, and the C-terminal region of p300 (0.5 μg each) and E1A (1.0 μg) were mixed, incubated in vitro on ice for 2 h, and immunoprecipitated, followed by Western immunoblotting using antibodies as shown. Sources of antibodies are as indicated in legends to Fig. 1D and 5A; antibody abbreviations are as in the legend to Fig. 5. For input lanes, proteins at 1/20 of the amounts used for the assays were loaded directly.