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Preformed p300-E1A complex still allows transient association of YY1 or HDAC3 with the complex. (A to D) p300 and E1A were preincubated on ice for 2 h to form a complex, and then either GST (A and C) or YY1 (B) and HDAC3 (D) were added. At the indicated time points, the proteins were immunoprecipitated (IP) using anti-p300 antibodies and Western immunoblotted (WB) with antibodies as shown. For antibody abbreviations, see the legend to Fig. 5.
