TABLE 2.

Analysis of B-cell and T-cell responses to MHV68 in control C57BL/6 and p50^−/− mice^a

dpi	Mean (SD) %b
	Germinal center B cells		Effector CD4+ T cells		Effector CD8+ T cells		Vβ4+ CD8+ T cells
	Control	p50−/−	Control	p50−/−	Control	p50−/−	Control	p50−/−
0 (naïve mice)	0.5	0.6	15.2 (3.2)	21.7 (5.2)*	2.8 (0.4)	3.9 (1.7)	3.9 (0.2)	3.8 (0.3)
18	5.3 (3.6)	0.7 (0.3)*	32.2 (3.9)	17.1 (2.3)*	27.2 (5.0)	6.4 (1.4)*	9.0 (3.3)	4.0 (0.2)
28	8.9 (1.9)	2.5 (1.4)*	38.3 (1.8)	28.6 (2.0)*	36.2 (20.2)	19.8 (9.5)	19.5 (14.7)	10.9 (6.5)*
45-56	3.6 (0.8)	0.7 (0.8)*	19.3 (4.4)	23.6 (2.2)	28.9 (7.3)	12.2 (3.4)*	23.3 (5.3)	8.8 (1.4)*
105	NA	NA	23.2 (6.3)	22.2 (6.0)	32.7 (9.5)	5.1 (1.4)*	31.0 (9.8)	5.7 (0.9)*

^aMice were infected with 100 PFU of MHV68 by intranasal inoculation. Specific cell subsets were derived from initial FACS gating on live lymphocytes.

^bThe data shown are the percentage for each subset derived from FACS analysis of three to five individual infected mice or naïve mice pooled from the individual harvest days. *, significant difference (P < 0.05) between infected control and p50^−/− mice. B cells were analyzed at 18, 28, and 56 dpi. T cells were analyzed at 18, 28, 45, and 105 dpi. NA, not analyzed. Germinal center CD19⁺ B cells were gated as percent CD95⁺/GL7+ of CD19⁺ B cells, effector CD4⁺ T cells were gated as percent CD62L^lo/CD44^hi of CD4⁺ T cells, effector CD8⁺ T cells were gated as percent CD62L^lo/CD44^hi of CD8⁺ T cells, and Vβ4⁺ CD8⁺ T cells were gated as percent Vβ4⁺ of CD8⁺ T cells.