FIG. 3.

Identification and verification of the yeast glycoprotein that cross-reacts with MAb 2G12. (A) The amino acid sequence of the glycoprotein Pst1, isolated from Δpmr1 Δmnn1 yeast and identified by nano-LC/MS/MS, is depicted. The detected peptides are underlined, with each being identified at least twice; the boldface letters at the N and C termini depict the signaling peptide and GPI anchor signal, respectively; and the highlighted letters are potential N-linked glycosylation sites. The amino acid sequence was obtained from GenBank under the accession number NP_010340 (23). (B) Pst1 was precipitated from Δpmr1 and Δpmr1 Δmnn1 culture media by 2G12 and detected by Western blotting with anti-Pst1. Starting material (S), flowthrough (F), wash (W), and eluate (E) samples are indicated.