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Analysis of Pst1 glycans. (A) MALDI-TOF mass spectrogram of N-linked glycans released from purified Δpmr1 Δmnn1-Pst1. (B) Δpmr1 Δmnn1-Pst1 digested with Endo H or with jack bean or A. saitoi mannosidase was analyzed by Western blotting using anti-Pst1 (left) or 2G12 (right), with mock-digested and untreated Pst1 used as controls.
