FIG. 5.
Contribution of GLUT-1 to HTLV-3 SU binding. (A) The cell surface levels of HSPG, NRP-1, and GLUT-1 on U87 clones C5 (GLUT-1+) and C8 (GLUT-1Low) were determined by flow cytometry, as in Fig. 2A. Black line, specific antibodies; gray line, isotype control. The result shown here is representative of four independent experiments. (B) Levels of binding of HTLV SU to C5/U87 and C8/U87. Data shown were calculated as for Fig. 4. An asterisk denotes significant differences from binding of the same SU to the C5/U87 cells. P values were as follows: HTLV-3 SU C5/U87 versus C8/U87, >0.05; HTLV-1 SU C5/U87 versus C8/U87, >0.05; HTLV-2 SU C5/U87 versus C8/U87, <0.001.