FIG. 10.

KSHV requires a low-pH environment for infection. HMVEC-d (A) and HUVEC (B) cells were left untreated or pretreated with nontoxic concentrations of bafilomycin (Baf) or NH₄Cl for 1 h at 37°C, washed, and infected with KSHV at an MOI of 10 (10 DNA copies/cell), and total RNA isolated at the indicated time points. Fifty nanograms of DNase-treated RNA/μl was subjected to real-time RT-PCR as described in the Fig. 2A and 5D legends. Histograms depict the percent inhibition of KSHV ORF73 and ORF50 gene RNA copy numbers in the presence of indicated nontoxic concentrations of drugs, calculated in comparison to copy numbers in cells incubated with virus alone. Each reaction was done in duplicate, and each bar represents the mean ± standard deviation of the results of three independent experiments.