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. 2009 Mar 4;83(10):5282–5288. doi: 10.1128/JVI.02485-08

FIG. 2.

FIG. 2.

Effects of expression of bat CoV nsp1 on reporter gene expression. 293 cells were independently cotransfected with pRL-SV40 and one of the following plasmids: pCAGGS (empty vector [EV]), pCAGGS-Nsp1-WT (WT), pCAGGS-Nsp1-mt (mt), pCAGGS-Rm1 (Rm1), pCAGGS-133 (133), or pCAGGS-HKU9-1 (HKU9-1). At 20 h posttransfection, cell extracts were prepared. (A) Western blot analysis using anti-myc monoclonal antibody (Upstate) and anti-β-actin antibody (Santa Cruz Biotechnology) demonstrated the accumulation of expressed nsp1 proteins and β-actin, respectively. The data are representative of results from three independent experiments. (B) RLuc activities were measured and represented as the average of results from three independent experiments. Activities are expressed in relative light units. (C) The abundance of expressed RLuc RNA transcripts from pRL-SV40 was examined by Northern blot analysis using an RLuc probe. The data are representative of results from three independent experiments. The abundance of 28S and 18S rRNAs in each sample is also shown.