FIG. 9.

VP22 colocalization with gE in infected cells is specific to its gE interaction domain. (A) Vero cells were infected with HSV-1 expressing GFP-22 or GFP-ΔgEbind at a multiplicity of 5 and examined live by fluorescence microscopy 8 h after infection. (B) Vero cells were infected with HSV-1 expressing GFP-22 or GFP-ΔgEbind at a multiplicity of 5 and fixed with 4% paraformaldehyde 8 h after infection. An immunofluorescence assay was carried out with the anti-gE antibody (red), and nuclei were stained with DAPI (blue).