Figure 5.

Western blot of explant culture medium. Protein from conditioned media after 24 h of culture was immunoreacted with a polyclonal antibody to human glycodelin. Treatment with hCG markedly increased the synthesis and the molecular size of glycodelin (lanes 3–6). Endometrial explants from the heat-inactivated controls secreted a low molecular weight form of glycodelin (lanes 7–10) comparable to that seen normally at day 10 PO (lane 2). Lane 1 is the human protein (0.25 μg) purified from first-trimester cytosolic extracts.