TABLE 1.
Rex-2 mutants and their Tax transactivation activities
| Rex-2 mutantsa | Tax-2 mutation(s) | Steady-state ratio of Tax transactivation activityb
|
|
|---|---|---|---|
| CREB/ATF | NF-κB | ||
| S151, S153A | L131P, F133R | 0.25 | <0.05 |
| S151D | L131G, A132S | 0.50 | <0.05 |
| S153D | F133G | 0.44 | <0.05 |
| P152D | A132G | 0.45 | 0.86 |
| A157D | wt | 1.0 | 1.0 |
| S151Term | wt | 1.0 | 1.0 |
| S158Term | wt | 1.0 | 1.0 |
a
The Rex-2 mutants generated were named based on the mutated amino acid and its position within the 170-amino-acid HTLV-2 Rex. For example, S151D indicates that the serine residue at amino acid 151 was substituted with aspartic acid.
b
Tax transactivation activities toward CREB/ATF- and NF-κB-responsive luciferase reporter genes were examined using the dual luciferase assay described in Materials and Methods. The data are based on three independent experiments and normalized to wt Rex-2 activity (set as 1.0).