TABLE 2.
Detection and quantification of HTLV-1 DNA in rabbit PBMCs
| Inoculum and rabbit no. | Presence of amplified PCR fragment (copy no., 102) at indicated wk postinoculationa
|
|||||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 4 | 8 | 11 | |
| 729/HTLV-2 | ||||||
| R1 | − | + | + (0.29) | + (1.74) | + (1.36) | + (1.20) |
| R2 | − | + | + (0.23) | + (0.48) | + (0.35) | + (0.83) |
| R3 | − | + | + (0.24) | + (0.54) | + (0.9) | + (0.53) |
| R4 | − | + | + (1.05) | + (0.32) | + (1.5) | + (3.28) |
| R5 | − | + | + (0.55) | + (0.36) | + (0.67) | + (1.07) |
| R6 | − | + | + (0.83) | + (0.4) | + (0.09) | + (0.42) |
| 729/HTLV-2 A157D | ||||||
| R7 | − | + | + (0.16) | + (1.27) | + (0.41) | + (1.12) |
| R8 | − | + | + (0.15) | + (0.69) | + (0.35) | + (1.22) |
| R9 | − | + | + (0.08) | + (0.44) | + (0.33) | + (0.20) |
| R10 | − | + | + (0.26) | + (2.76) | + (0.27) | + (1.25) |
| R11 | − | + | + (0.22) | + (1.37) | + (0.28) | + (0.76) |
| R12 | − | + | + (0.93) | + (1.57) | + (1.55) | + (2.20) |
| 729/HTLV-2 S158Term | ||||||
| R13 | − | + | + (0.14) | + (0.30) | + (0.26) | + (0.94) |
| R14 | − | + | + (0.47) | + (0.47) | + (1.76) | + (4.31) |
| R15 | − | + | + (0.47) | + (4.74) | + (0.91) | + (1.23) |
| R16 | − | + | + (0.05) | + (1.52) | + (0.13) | + (0.46) |
| R17 | − | + | + (0.24) | + (0.30) | + (3.48) | + (4.16) |
| R18 | − | + | + (0.05) | + (0.42) | + (0.27) | + (0.81) |
| 729 | ||||||
| R19 | − | − | − (0.05) | − (0.05) | − (0.01) | − (0.00) |
| R20 | − | − | − (0.03) | − (0.03) | − (0.04) | − (0.03) |
a
Genomic DNA isolated from rabbit PBMCs was subjected to PCR and to real-time TaqMan PCR analyses (0, 2, 4, 8, and 11 weeks) using HTLV-2-specific primers (670/671). −, no amplified PCR fragment; +, amplified PCR fragment. As determined by ANOVA, copy numbers in rabbits inoculated with the mutant proviruses were not significantly different from those of the wt. P values for weeks 2, 4, 8, and 11 are 0.216, 0.517, 0.498, and 0.443, respectively.