TABLE 1.
Vectors and primers used to generate the UL31 subclones
| Plasmid | UL31 codon (aa)a | Vector(s) | Primersb |
|---|---|---|---|
| UL31(dN)-pCDNA3 | 45-306 | pcDNA3 | 5′-ATACTCGAGATGCCGCCTCACG-3′, 5′-TATACTCGAGCTACGGCGGAGGAAAC-3′ |
| UL31-Flag | 1-306 | pBudCE 4.1 | 5′-ATACTCGAGACCTATGTATGACACCGACCC-3′, 5′-ATAAGATCTTACTTATCGTCGTCATCCTTGTAATCCGGCGGAGGAAACTC-3′ |
| UL31(dN)-Flag | 45-306 | pBudCE 4.1 | 5′-ATACTCGAGACCTATGCCGCCTCACG-3′, 5′-ATAAGATCTTACTTATCGTCGTCATCCTTGTAATCCGGCGGAGGAAACTC-3′ |
| GST-UL31 (1-50 aa) | 1-50 | pCR2.1, pGEX4T-2 | 5′-ATGTATGACACCGACCCCCATC-3′, 5′-TTTGCGGGCGTGAGGC-3′ |
| GST-UL31 (52-165 aa) | 52-165 | pCR2.1, pGEX4T-1 | 5′-TCTCGAGATGGAGGAGCTGTGTTTAC-3′, 5′-TCTAGGCCAACACGACCAA-3′ |
| GST-UL31 (159-265 aa) | 159-265 | pCR2.1, pGEX4T-1 | 5′-GCTCGAGATGGTGGCCTCCTTGGTC-3′, 5′-CCTAGTCCGTGGGTTTCTCTGCGTT-3′ |
| GST-UL31 (255-306 aa) | 255-306 | pCR2.1, pGEX4T-1 | 5′-CCTCGAGATGGGCAACGCAGAGAAACC-3′, 5′-CCTACGGCGGAGGAAACTC-3′ |
| GST-UL31 (SA3) | 1-306 | pGEX4T-1 | 5′-TAGAATTCATGTATGACACCGACCCCCATC-3′, 5′-TATACTCGAGCTACGGCGGAGGAAAC-3′ |
| GST-UL31 (SA6) | 1-306 | pGEX4T-1 | 5′-TAGAATTCATGTATGACACCGACCCCCATC-3′, 5′-TATACTCGAGCTACGGCGGAGGAAAC-3′ |
a
aa, amino acids.
b
Restriction sites used for cloning are underlined, and Flag sequences are in bold.