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. 2009 Mar 11;83(10):5256–5268. doi: 10.1128/JVI.01997-08

FIG. 1.

FIG. 1.

Lymphocyte cellularity in the draining PLN following acute infection with HSV-1. C57BL/6 (WT) mice, CD4-depleted B6 mice, or B6-MHC-II−/− mice were infected in each hind FP with 2 × 106 PFU HSV-1. Mice were sacrificed 5 days p.i., and the draining PLN cells were analyzed by flow cytometry. (A) Absolute number of HSV-1 gB-specific CD8+ T cells (four mice/group; *, P = 0.03). (B) Absolute number of HSV-1 gB-specific CD8+ T cells (three mice/group; *, P = 0.04). (C) Absolute number of total cells per PLN (four mice/group; *, P < 0.0001). (D) Absolute number of total cells per PLN (three mice/group; *, P = 0.0001). (E) Absolute number of CD8+ T cells per PLN (four mice/group; *, P < 0.0001). (F) Absolute number of CD8+ T cells per PLN (three mice/group; P = 0.2). For panels A and B, lymph node cells were simultaneously stained with anti-CD8α and H-2Kb-gB498-505 tetramers to identify HSV-1-specific CD8+ T cells. Data are representative results from one of two independent experiments for each group (means ± SEM).