FIG. 8.

Selected resistance mutations to maC46 reduce the susceptibility to soluble CFI. PM-1 cells were challenged with replication-incompetent lentiviral vectors. The viral particles were pseudotyped with the indicated Env constructs and encoded an eGFP marker gene. The amino acid substitutions within the BaL Env mutants are given in Fig. 3. The single-round infection assay was carried out in the presence of increasing concentrations of T-20 (A), C46 (B), or T-1249 (C) (Fig. 1). Five days later, eGFP expression was determined by FACS. Error bars represent the standard deviations of triplicates. Nonlinear regression (variable slope) was performed with GraphPad Prism (version 5). Top and bottom constraints were set to equal 100% and 0%, respectively.