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. 2009 May 15;877(14-15):1516–1522. doi: 10.1016/j.jchromb.2009.03.036

Fig. 4.

Fig. 4

Dot-blot of recombinant protein antigens reacted with antibody pools from sheep immunised with EG95–GST. 1 μg of each protein was spotted onto the nitrocellulose membrane. Lane A is pooled serum from sheep immunised with EG95–GST. Lane B is the pre-immune pool. Lane C is reacted with IgG affinity-purified to conformational epitope/s of EG95 (anti-cEG95). Lane D is reacted with IgG affinity-purified to GST (anti-GST). Lane E is reacted with antibody affinity-purified to E100 peptide (anti-E100). Lane F is reacted with antibody affinity-purified to G1 peptide (anti-G1).