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Western Blot. Recombinant proteins EG95–MBP (lane 1), EG954–74 (lane 2), EG9554–109 (lane 3), and EG9592-156 (lane 4) probed with three antibody sources. Pooled sera from sheep vaccinated with EG95–GST (Panel A), polyclonal antibodies affinity purified to conformational epitopes of EG95 (anti-cEG95) (Panel B) and polyclonal antibodies affinity purified to peptide E100 (Panel C). A Coomasie stained SDS-PAGE displaying recombinant proteins.
