Skip to main content
. 2009 May 29;5(5):e1000460. doi: 10.1371/journal.ppat.1000460

Figure 1. Generation of Δrv2623 M. tuberculosis strain.

Figure 1

(A) Genomic organization of the rv2623 gene locus. Genes appear as large arrows in their native orientation. Small arrows represent forward and reverse primers used for long range PCR. Sizes of the rv2623 deletion and hyg-insertion are indicated. The location of the radiolabeled probe (black bar) as well as relevant Sph1 sites (S) is indicated as they pertain to the Southern blot. (B) An autoradiograph of the Southern blot shows radiolabeled Sph1 fragments from the wild type (lane 1) and Δrv2623 strain (lane 2). The sizes indicated represent those expected for wild type and the deletion mutant.