Figure 5. M. tuberculosis Rv2623 is a nucleotide-binding USP.
High Performance Liquid Chromatography (HPLC) analysis of endogenously bound nucleotides from purified His6-Rv2623. Nucleotides species were identified based on their specific retention times on the Mono Q HR 5/5 column, represented by peaks in absorbance at 260 nm, which correspond to that of nucleotide standards (not shown). Bound nucleotides were extracted by boiling, and separated and quantified from a standard curve that relates absorbance peak area to the known amount of ATP injected onto the column (inset).