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. 1999 Mar 2;96(5):2549–2553. doi: 10.1073/pnas.96.5.2549

Figure 2.

Figure 2

Western immunoblot analysis of AlMV CP expression in plants infected with Av/A4. Proteins were separated electrophoretically on a 13% SDS-polyacrylamide gel, transferred to a membrane, and reacted with mAbs specific for AIMV CP. (A) Antibodies recognized a 24-kDa protein in samples from locally (L) and systemically (S) infected tissue collected from N. benthamiana, N. tabacum MD609, and S. oleracea. (B) Antibodies also reacted with the 24-kDa protein in extracts from tissue locally infected (lane L) with Av/CP+P. Av/CP+P was not detected in upper uninoculated leaves (lane S). Left lanes show AlMV CP from wild-type virus (positive control) and the absence of CP in an Av-infected plant sample (negative control), respectively.