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. 1999 Mar 2;96(5):2549–2553. doi: 10.1073/pnas.96.5.2549

Figure 3.

Figure 3

Northern analysis (A) and RT-PCR (B) of viral RNA isolated from leaves of plants systemically infected with Av/A4. RNA molecules were separated by electrophoresis in denaturing conditions, transferred to a membrane, and probed with minus-strand RNA resembling 250 5′ nt of the TMV genome. In vitro synthesized transcripts of Av/A4 were used as a positive control (lane 5). Lanes 1 and 2 show RNA purified from systemically infected leaves of N. benthamiana and N. tabacum MD609, respectively. Total RNA purified from the upper uninoculated leaves of N. benthamiana (lane 3) and N. tabacum MD609 (lane 4) infected with Av/CP+P was used as a negative control. (B) RT-PCR of total RNA confirmed the presence of the full-size AlMV RNA4 insert in recombinant Av/A4 (lane 3). In vitro synthesized transcripts of Av (lane 2) and total RNA purified from upper uninoculated leaves of N. benthamiana infected with Av/CP+P were used as negative controls (lane 1).