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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Bioconjug Chem. 2009 Feb;20(2):213–221. doi: 10.1021/bc800237t

Figure 5.

Figure 5

Triplex formation with target duplex DNA after release from M6P-GFLG-HPMA-GFLG-32P-TFO conjugate. Samples were applied on 15% native PAGE at 4 °C in 89 mM Tris-borate buffer, containing MgCl2 (20 mM) for 4 h. Lane 1: 32P-TFO; lane 2: duplex DNA/32P-TFO (200:1) incubated for 24 h; lane 3: Duplex DNA/32P-TFO (200:1) and papain incubated for 24 h; lane 4: M6P-GFLG-HPMA-GFLG-32P-TFO; lane 5: M6P-GFLG-HPMA-GFLG-32P-TFO incubated with papain for 24 h at 37 °C; and lane 6: duplex DNA/M6P-GFLG-HPMA-GFLG-32P-TFO (200:1) incubated for 24 h at 37 °C with papain.