The effects of CEES on markers of cellular oxidative stress and prevention
by AEOL 10150 in 16HBE cells. Cells exposed to 900 μM CEES for 12 h had
decreased total cellular GSH levels (A), and AEOL 10150 (50 μM) rescued
this decrease when treated 1 h after CEES exposure. Total GSH levels were
normalized to the amount of protein and expressed as nanomoles of GSH per
milligram of protein. CEES also increased the levels of the DNA oxidation
marker 8OHdG (B), and AEOL 10150 (50 μM) post-CEES treatment decreased the
levels of DNA oxidation. Data expressed as a ratio of 8OHdG per 105
2dG. Data presented as mean ± S.E.M., n = 4 to 8;
*, p < 0.05; ***, p < 0.001
compared with control levels. Two-way ANOVA of AEOL 10150, p =
0.1444; CEES, p = 0.0001; interaction, p = 0.0481 (A); and
AEOL 10150, p = 0.1394; CEES, p < 0.0001; interaction,
p = 0.0004 (B).