Fig. 1.
Reduced striatal surface DAT expression in KO compared with WT mice. A, representative Western blot of the striatal surface and total DAT expression for WT and KO mice. Striatal synaptosomes were biotinylated, and bands were quantified to determine the surface and total DAT expression as described under Materials and Methods. KO mice showed less surface (biotinylated) DAT level than WT mice (Student's t test, p < 0.05, n = 6), whereas no significant genotype difference in the expression of the total DAT was observed. B, surface DAT was also compared between genotypes by determining binding of the DAT antagonist [3H]WIN 35,428 with striatal synaptosomes. Striatal synaptosomes from WT and KO mice were incubated with different concentrations of [3H]WIN35,428 for 1 h at 4 °C. Bmax was significantly lower for KO mice than for WT mice (Student's t test, p < 0.05, n = 5-6), whereas Kd values did not differ. Data are represented as mean ± S.E.M.