Fig. 4.

E2 protection against EW-induced MMS. HT22 cells were exposed to control media or ethanol (100 mM) for 24 h and withdrawn for 4 h. E2 (0.1 or 1 μM) was simultaneously treated with ethanol and readministered when ethanol-containing media were replaced with control media at the end of the ethanol exposure. Cells were collected at 4 h of EW (EW cells) for assessment of MMS. EW cells showed more rapid absorbance decline (more MMS) than cells treated with control (P < 0.001), 1 μME2 (P < 0.001), or 0.1 μME2 (P < 0.05). Depicted are mean ± S.E.M. for n = 3/group. Some S.E.M. values were too small to be depicted.