Fig. 5.

E2 treatment window for E2 protection. HT22 cells were exposed to control media or ethanol (100 mM)-containing media. Ethanol-treated cells were further divided into three groups: 1) ethanol + control media, 2) E2 treatment (1 μM) during the entire period of ethanol exposure and EW, and 3) E2 treatment that was restricted to the EW phase. Cells were collected at 4 h of EW to assess MMS. EW cells had the most rapid MMS (absorbance decline) among treatments (P < 0.001). E2 treatment that was restricted to the EW phase significantly delayed (P < 0.05) MMS toward control cells or toward EW cells that were treated with E2 during ethanol exposure and EW. No difference was observed between E2 treatments during the two different time windows. Depicted are mean ± S.E.M. for n = 3/group. Some S.E.M. values were too small to be depicted.