Fig. 3.

A, effect of cotransfection of HNF1α and HNF1β on CNT2 basal promoter function in HepG2, HCT116, and ACHN cells. B, stimulatory effects of HNF1α and HNF1β on CNT2 haplotype promoter constructs in HepG2 cells. In both experiments, the cells were transfected with 200 ng of CNT2 haplotype promoter constructs (-265/+100 reference) or the promoterless pGL4.11b vector with 100 ng of pCDNA3.1(+) vector alone or with 100 ng of pcDNA3.1(+) vector containing HNF1α or HNF1β and with Renilla constructs (8 ng). The cells were assayed for luciferase activity after 24 h. The results here are shown as the mean ± S.D. of two separate experiments performed in triplicate. ^**, p < 0.01; ^***, p < 0.001. Multiple comparisons were analyzed using one-way analysis of variance followed by Dunnett's two-tailed test. CNT2 basal promoter reference (TTA-haplotype) was used as control.