TABLE 1.

Sequences of oligonucleotides for functional assays and cloning

Underlined nucleotide sequences in the primer pairs for cloning represent the specified restriction enzyme sites.

	Oligonucleotide Sequences
Site-Directed Mutagenesis Experiment
rs60273882 (− 241C>T)	5′-GTCTCAAGCGATCTGCCCGTCTCGGCC-3′
rs2413775 (− 146A>T)	5′-CCTGCAAAACAGTCTTAAAAACATATAATATTTAACTTGAGAGGTGCA-3′
rs2899376 (− 115G>T)	5′-GGTGCAGTCCTCCTCTACATTGAGGGCAGG-3′
rs60354957 (− 14A>T)	5′-CCTCCCTCGCAGCTGTGCTTTTCTTTCAGTC-3′
rs61553676 (− 1G>A)	5′-CTCGCAGCTGAGCTTTTCTTTCAATCCTTCACTGAG-3′
EMSA probes and competitors
−146-A	5′-GGGCTTAAAAACATATAA TAATTAACTTGAGAGGTGCAGTC −3′
−146-T	5′-GGGCTTAAAAACATATAATATTTAACTTGAGAGGTGCAGTC −3′
HNF1α per	5′-GGGCGCAAAAGAAAGTTAATCATTAACCCGGGAAACAGC-3′ (Kikuchi et al., 2006)
Cloning
CNT2 basal promoter	5′-ATAACGCGTTATCCTGGCTGGTCTCAAGCG-3′ (MluI)
(−265/+ 100)	5′-ATAAGATCTATCTCTTTAAAAATTTGTAAAT-3′ (BglII)
HNF1α	5′-AAGCTTGCCATGGTTTCTAAACTGAGCC −3′ (HindIII)
	5′-GAATTCTGGTTACTGGGAGGAAGAGGCC −3′ (EcoRI)
HNF1β	5′-AAGCTTGAAAATGGTGTCCAAGCTCACG −3′ (HindIII)
	5′-GAATTCGGCATCACCAGGCTTGTAGAGG −3′ (EcoRI)