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Colon cell proliferation determined by BrdU labeling index and apoptosis evaluated by TUNEL staining. All pictures are in 40× magnification. A, BrdU staining in the colon, a BrdU-positive cell is a cell with nuclei stained brown. B, TUNEL staining of the colon. C, quantification of the percentage of BrdU positively stained cell (LI). D, quantification of the percentage of TUNEL positively stained cells. *, P < 0.05.
