Expression of genes involved in cell proliferation, tumor suppression, and
inflammation in the colons of 2- and 12-month-old male WT and FXR KO mice. A,
mRNA levels of genes in 2-month-old male mice. B, mRNA levels of genes in
12-month-old male mice. n = 6/genotype/time point. *, P <
0.05. C, Western blot analysis of cyclin D1, c-Myc, β-catenin, and APC
protein in the colon of 2- and 12-month-old WT and FXR KO male mice, with
n = 6 per group. D, immunohistochemistry staining of β-catenin
in the colon of 2- and 12-month-old WT and FXR KO male mice.