TABLE 2.

Comparison of effects of agonists and antagonists on functional responses from native and recombinant α3β4 nAChRs

	Recombinant Rat α3β4 nAChRsa ([Ca2+]i) EC50 or IC50	Native Bovine α3β4* nAChRs (Neurosecretion) EC50 or IC50
	μM
Nicotine	18.0 (15.9–20.4)	3.6 (2.9–4.5)b
Epibatidine	0.06 (0.05–0.07)	0.009 (0.006–0.014)b
d-Tubocurarine	6.3 (5.0–8.1)	2.2 (1.4–3.6)c
Mecamylamine	0.6 (0.4–0.8)	0.1 (0.1–0.7)d
Tetracaine	2.5 (2.4–2.6)	4.0 (2.2–7.4)d
COB-2	1.1 (1.0–1.1)e	1.2 (1.2–1.3)e

^aData are means (confidence limits) of three to four different experiments performed in triplicate or quadruplicate

^bEC₅₀ value from Wenger et al. (1997)

^cIC₅₀ value from McKay and Burkman (1993)

^dIC₅₀ value from McKay and Trent-Sanchez (1990)

^eIC₅₀ value from McKay et al. (2007) and Table 1