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. Author manuscript; available in PMC: 2009 May 14.

Published in final edited form as: Biochim Biophys Acta. 2007 Jul 6;1769(9-10):546–558. doi: 10.1016/j.bbaexp.2007.06.004

The expression of ITGA1, ITGA2, PELO and the housekeeping gene HPRT in human cell lines. (A) The level of mRNA in each cell line was measured semi-quantitatively using limiting cycle RT-PCR. For each of the four mRNAs, the cDNA product obtained after 27 or 29 cycles was separated by agarose gel electrophoresis and visualized with ethidium bromide. Comparisons were made between cells cultured in the presence (+) of 5 nmol/L phorbol myristate (PMA) or an equal volume of DMSO (−) for 24 hours. Total RNA was reverse transcribed and the cDNA obtained was used for PCR amplification. Both the megakaryocytic (MK) cell lines HEL, K562, Dami, and CHRF-288-11 and the non-MK cell lines C8161 and HeLa were studied.

The expression of ITGA1, ITGA2, PELO and the housekeeping gene HPRT in human cell lines. (A) The level of mRNA in each cell line was measured semi-quantitatively using limiting cycle RT-PCR. For each of the four mRNAs, the cDNA product obtained after 27 or 29 cycles was separated by agarose gel electrophoresis and visualized with ethidium bromide. Comparisons were made between cells cultured in the presence (+) of 5 nmol/L phorbol myristate (PMA) or an equal volume of DMSO (−) for 24 hours. Total RNA was reverse transcribed and the cDNA obtained was used for PCR amplification. Both the megakaryocytic (MK) cell lines HEL, K562, Dami, and CHRF-288-11 and the non-MK cell lines C8161 and HeLa were studied.