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. Author manuscript; available in PMC: 2009 May 14.
Published in final edited form as: Biochim Biophys Acta. 2007 Jul 6;1769(9-10):546–558. doi: 10.1016/j.bbaexp.2007.06.004

FIGURE 9.

FIGURE 9

Inhibition of ITGA1 promoter activity by in vitro methylation. The ITGA1 promoter construct (−397/+455) and pGL2b vector alone (control) were methylated in vitro with SssI methylase and transfected into Dami cells. Co-transfection of PRL-TK plasmid was used to normalize for transfection efficiency. The relative Luciferase activity for each transiently transfected cell preparation was measured and is plotted on the ordinate The activity of each of four constructs (abscissa) is compared: pGL2b; methylated pGL2b (Met-pGL2b); ITGA1 promoter (−397); and methylated ITGA1 promoter (Met-397).