Fig. 7.

Ionic strength and pH solution modifications do not alter PEG-induced isotherm variations on total cell lipid extract monolayer. Total LLC-PK1 cell lipid extract was applied on Milli-Q water subphase or on EC solution subphase and monolayer compression was performed at 37°C without (zero reference) or with 1 g/L of PEG 20,000 Da or PEG 35,000 Da. Surface pressure/mean molecular area isotherms were obtained as described in the “Materials and methods” section. Relative modification from pure Milli-Q water subphase was expressed as the percentage of variation in surface pressure measured at the collapse-point. Relative modification from pure Milli-Q water subphase was expressed as the percentage of variation in mean molecular area (mma) measured at the lift-off point (a) or as the percentage of variation in surface pressure (Π) measured at the collapse-point (b). Results are means ± SD of two independent experiments