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. 2009 Apr 3;60(7):2035–2044. doi: 10.1093/jxb/erp076

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Histochemical staining to test the activity of the RbCP1 promoter using a translational promoter–GUS fusion construct by agroinfiltration. Petals in intact buds were infiltrated at the centre of the petal with agrobacteria containing different constructs (three flowers per construct and three petals per flower) using a syringe. Buds were kept for 2 d on the plant and then cut under water as described and treated with ethylene for 8 h before GUS staining. The constructs used for GUS expression study were pBI101 (promoterless), pBI121 (GUS driven by the CaMV 35S promoter), pHAESA (GUS driven by the 1.6 kb abscission-specific promoter of the Arabidopsis HAESA gene), and pRbCP1 (GUS driven by the 2.0 kb rose RbCP1 promoter). GUS expression in the tips of the petals (at the point of separation from the thalamus) is shown. White arrows indicate the point of separation of the petal from the thalamus. (This figure is available in colour at JXB online.)