Table 1.
Sequence analysis and comparison of banana PME genes
| (A) | ||||||||
| Sequence | Gene or cDNA length (bp) | Polypeptide length (aa) | MW (kDa) | pI | Promoter length | Exon 1 | Intron | Exon 2 |
| MaPME1 | 1923 | 565 | 61 | 7.8 | – | – | – | – |
| MaPME2 | 3365 | 559 | 61 | 6.7 | 1162 | 960 | 68 | 720 |
| MaPME3 | 3186 | 574 | 62 | 7.06 | 1000 | 1029 | 81 | 696 |
| (B) | ||||
| Polypeptides | MaPME1: 1845 bp (565 aa) |
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| Length | % identity (similarity) | Scov | Qcov | |
| MaPME1 | 565 | – | – | – |
| MaPME2 | 559 | 46 (60) | 81.06 | 81.93 |
| MaPME3 | 574 | 47 (59) | 97.17 | 95.64 |
| Nucleotides | CDS |
|||
| Length | % identity | Scov | Qcov | |
| MaPME1 | 1895 | – | – | – |
| MaPME2 | 2135 | 75 | 9.3 | 8.2 |
| MaPME3 | 2104 | 75 | 27.7 | 24.9 |
Analysis of nucleotide and polypeptide sequences of PME genes isolated in this study. The features of nucleotide and polypeptide sequences of MaPME1 cDNA and those of the two genomic sequences of MaPME2 and MaPME3 are given in (A). A comparison of nucleotide and polypeptide sequences of MaPME genes (B), including determination of Scov and Qcov values, was performed using BLAST 2 (Tatusova and Madden, 1999).