Table 2.

Metabolic analysis of the lines derived from the cross between VilB and Cervil parents

	Harvest 2004						Harvest 2005
	B1	B2	B4	B9a	B9b	CxB	B1	B2	B4	B9a	B9b	Bx
β-Alanine	0.95	1.04	0.68	1.64	1.18	0.51	1.34	4.06	0.79	1.34	1.39	2.21
Alanine	2.96	2.37	0.79	2.32	1.59	2.41	1.24	1.16	1.09	2.17	1.84	0.69
Asparagine	0.83	1.51	0.71	0.97	1.10	1.46	3.19	1.63	1.16	0.73	0.92	1.27
Aspartate	0.76	0.80	0.85	1.03	0.70	0.61	0.87	0.55	0.88	1.13	0.94	0.74
Cysteine	0.94	1.33	0.78	1.57	nd	0.29	0.77	0.90	0.77	0.69	0.61	0.58
γ-Aminobutyrate	0.88	0.92	0.92	1.15	0.79	0.61	0.61	0.91	0.57	0.61	0.69	0.44
Glutamate	1.34	0.97	0.92	1.08	0.67	1.51	1.04	1.00	0.96	1.12	1.20	1.08
Glutamine	0.92	1.09	0.67	1.04	0.95	nd	1.09	1.02	0.95	0.95	1.01	0.95
Glycine	0.78	1.22	0.66	1.20	1.49	0.23	0.82	1.56	0.91	1.64	2.24	0.65
Homoserine	0.66	0.77	0.53	0.66	0.65	0.57	0.40	0.35	0.87	0.41	0.35	0.39
Isoleucine	0.55	0.92	0.73	0.70	0.79	0.59	0.76	1.18	0.96	0.94	1.14	0.76
Lysine	0.85	0.89	1.00	0.71	1.78	27.82	0.70	0.53	1.28	0.44	0.50	0.65
Phenylalanine	0.79	1.16	0.61	0.72	0.73	0.60	0.88	1.13	0.94	0.92	1.24	1.02
Proline	3.86	1.80	0.63	3.61	1.10	4.46	5.64	1.69	1.07	2.52	1.72	7.54
Putrescine	1.69	1.15	1.83	2.25	1.57	2.56	1.15	1.06	1.60	1.64	1.19	1.75
Pyroglutamate	1.13	1.00	0.92	1.04	0.88	0.55	1.04	0.97	1.03	1.04	1.03	1.03
Serine	0.77	1.07	0.90	0.99	0.99	0.37	0.79	1.19	0.82	0.91	1.19	0.59
Threonine	0.83	0.96	0.56	1.05	0.80	0.44	0.46	0.58	0.40	0.87	0.71	0.33
Valine	0.62	1.08	0.74	1.12	1.15	0.32	0.72	1.43	0.91	1.37	1.71	0.70
Fructose	1.20	1.03	1.04	1.08	0.82	0.93	1.09	0.92	0.94	1.02	1.03	1.09
Fructose-6-P	1.22	1.14	0.61	0.88	nd	0.77	1.27	1.01	1.06	1.16	0.69	1.93
Gentiobiose	1.97	1.31	1.03	1.20	1.18	0.70	1.16	0.96	0.76	1.21	1.04	1.05
Glucose	1.14	0.96	0.96	1.09	0.79	1.02	1.28	0.90	0.54	1.49	0.74	0.82
Glucose-6-P	1.20	1.11	0.65	1.01	1.03	0.68	1.45	1.31	0.99	1.30	1.12	1.42
Isomaltose	1.95	1.32	1.31	1.27	1.18	1.07	1.81	0.85	0.62	1.41	2.93	1.16
Maltose	1.49	1.00	0.53	0.61	5.37	0.73	1.27	0.93	1.37	0.96	1.25	1.50
Sucrose	1.63	1.82	0.68	2.44	1.82	2.25	1.73	1.24	0.79	1.68	1.38	1.77
Trehalose	1.46	1.02	0.97	1.39	2.11	0.83	1.93	1.01	1.16	2.01	6.39	1.33
Xylose	0.97	0.94	1.43	0.95	0.69	0.79	0.63	0.65	0.73	0.56	0.61	0.79
Glycerol	1.25	1.15	1.09	1.00	0.82	1.38	1.24	1.47	0.65	0.86	0.58	0.67
Myo-ino-1-P	1.31	0.96	0.81	1.01	0.94	nd	1.29	1.16	0.94	0.93	1.09	0.97
Myo-ino	0.70	0.69	0.56	0.99	1.06	0.38	0.99	0.84	0.72	1.03	1.07	0.95
Benzoate	1.87	1.13	1.38	2.26	1.81	0.90	1.15	1.43	1.34	1.01	1.02	1.14
Citramalate	0.90	0.73	1.18	0.85	0.72	0.86	1.29	1.08	1.10	1.30	1.27	1.26
Citrate	1.26	1.04	0.89	1.15	0.89	0.73	1.08	0.93	0.88	0.98	0.95	1.04
Gluconate	1.17	1.07	1.17	1.19	1.33	0.66	0.00	0.00	0.00	0.00	0.00	0.29
Glycerate	0.79	0.75	0.78	0.80	0.74	0.43	0.90	0.69	1.40	0.98	1.09	1.03
Malate	0.67	0.96	0.67	1.66	1.23	0.27	1.09	0.92	0.97	1.50	1.12	0.73
Nicotinate	1.30	1.03	0.99	1.05	0.90	1.63	0.92	0.81	0.74	1.06	0.88	0.91
Phosphorate	1.27	0.96	0.97	1.01	0.77	0.68	0.96	0.90	1.10	0.92	1.12	0.90
Saccharate	0.71	0.14	0.47	0.43	0.21	0.26	0.95	0.78	0.79	0.90	0.89	1.07
Succinate	1.16	1.16	0.83	0.94	1.00	1.68	1.71	1.20	0.94	0.81	1.27	1.91
16:00	1.20	1.03	1.04	0.99	1.69	0.38	0.59	0.71	1.41	1.23	0.87	0.76
18:00	1.19	0.95	0.94	0.91	0.98	0.36	1.04	0.90	1.02	1.18	0.92	1.09

Values are presented as the mean of six biologically independent determinations. The fold changes are relative to the VilB parent. In bold are those values which were significantly different with P <0.05 by the performance of Student's t-tests.