Table 3.

K⁺ and Cl^– concentrations in rice coleoptile tips after 48 h re-aeration (mM, tissue water basis, calculated from fresh weight:dry weight ratio and assuming 10% water in the free-space), for the experiments in which time trends on net K⁺ and Cl^– uptakes are shown in Fig. 4B

Tissue ions (mM)	Glucose at 2.5 mM during anoxic phase		Glucose at 50 mM during anoxic phase		l.s.d. (P <0.05)
After anoxic treatments and re-aeration	pH 3.5 60–108 h during anoxia	pH 6.5 continuous	pH 3.5 60–108 h during anoxia	pH 6.5 continuous
K+	143±12	163±16	137±24	165±5	26
Cl–	116±18	127±3	95±1.5	106±3	14

The tips had been at 2.5 mM or 50 mM glucose since transfer to anoxia. At 60 h anoxia, some tips were transferred from pH 6.5 to 3.5. At 108 h anoxia, the tips were transferred to aerated solution and then sampled after 48 h re-aeration. During anoxia, K⁺ was at 0.20–0.25 mM with no Cl^– in the basal medium as described in the Materials and methods. After the start of re-aeration, all treatments contained: K⁺ 0.4 mM, Cl^– 0.3 mM, and glucose 5 mM, in the basal medium. Values are means±standard errors of three replicates.