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. 2009 Apr 10;60(7):2129–2138. doi: 10.1093/jxb/erp091

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — NO signalling agonists promote the effects of ATPγS on pollen germination, and antagonists block the effects of ATPγS on pollen germination and elongation. Different classes of NO signalling agonists and antagonists and various concentrations of ATPγS were added to Arabidopsis WS pollen prior to germination. (A) Water-soluble NO signalling agonists (1 μM Viagra, 20 μM NONOate, 100 μM DibcGMP) shifted the concentration needed for inhibition of germination from 100 μM ATPγS to 50 μM. (B) DMSO-soluble NO signalling agonists (20 μM IBMX, 20 μM SNAP) shifted the concentration needed for inhibition of germination from 100 μM ATPγS to 50 μM. (C) ODQ (100 μM), a DMSO-soluble NO signalling antagonist, reverses pollen germination inhibition by ATPγS. Each agonist or antagonist was used at a concentration that by itself did not affect pollen germination. (D) ODQ also reverses the ATPγS inhibition of WS pollen tube elongation. Treatments were added to growing pollen tubes. Control is PGM+0.5% DMSO, ATPγS concentration is 100 μM+0.5% DMSO and ODQ concentration is 100 μM, which by itself had no effect on pollen tube growth. Different letters above the bars indicate values that are significantly different from each other (P ≤0.05). Error bars are ±SE.