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. 2009 Apr 8;60(7):2169–2178. doi: 10.1093/jxb/erp098

Fig. 2.

Fig. 2.

The effect of seed vernalization treatment on expression of HvVRN1. (A) Expression of HvVRN1 in seedlings at different time points (weeks) during seed vernalization. (B) RT-PCR analysis of HvVRN1 expression (35 cycles) in different organs during seed vernalization. Expression of ACTIN (25 cycles) is shown as a positive control, and two biological repeats are shown for each data point. NV refers to samples from non-vernalized plants and V refers to samples from vernalized plants. (C) Expression of HvVRN1 in the shoot apex at the third leaf stage, in non-vernalized plants (0) compared with plants that received seed vernalization treatments of different durations (1–9 weeks). (D) Expression of HvVRN1 in leaves (fully expanded second leaf) at the third leaf stage in the same experiment. (E) HvVRN1 expression in shoot apex in short versus long days at the third leaf stage, in non-vernalized plants (0) or after seed vernalization treatments of different durations (5, 7, or 9 weeks). (F) Comparison of HvVRN1 expression in leaves (fully expanded second leaf) in the same experiment. Expression of HvVRN1 was assayed by quantitative RT-PCR and is shown relative to ACTIN. ND denotes no expression detected. Asterisks indicate P-values of Student's t-test: ***P <0.001.