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. 2009 Apr 8;60(7):2169–2178. doi: 10.1093/jxb/erp098

Fig. 3.

Fig. 3.

The effect of seed vernalization treatment on HvVRN2 expression. (A) HvVRN2 expression in non-vernalized seedlings (control) versus seedlings germinated at low temperature for 7 weeks (cold). (B) Expression of HvVRN2 in the shoot apex or second leaf for non-vernalized plants at the third leaf stage, in short versus long days. (C) Relative expression of HvVRN2 in leaves (fully expanded second leaf) at the third leaf stage in long days, in non-vernalized plants (0), or after seed vernalization for different durations (1–9 weeks). (D) Relative expression levels of HvVRN2 in leaves (fully expanded second leaf) from plants at the third leaf stage in short or long days. The comparison shows non-vernalized plants (0) versus plants grown from seeds vernalized for 5, 7, or 9 weeks. Expression of HvVRN2 was assayed by quantitative RT-PCR and is shown relative to ACTIN. ND denotes no expression detected.