Fig. 5.

The effect of seed vernalization treatment on expression of HvFT1. (A) Plants subjected to different vernalization treatments were grown in long days until the third leaf stage when different organs were isolated for gene expression analysis. Expression of HvFT1 was assayed in the apex, second leaf, third leaf, or the sheath and compared with expression in whole plants. The comparison shows HvFT1 expression in control (non-vernalized) versus vernalized plants (9 weeks seed vernalization). Expression of HvFT1 was assayed by quantitative RT-PCR and is shown relative to ACTIN. ND denotes no expression detected.