Table 1. Incidence of zMPNSTs in zebrafish rp-heterozygous lines.
rp-heterozygous fish were collected as tumors became apparent, or as healthy fish at the maximum age specified (Age Range). Number Lost indicates those that either died before the appearance of external symptoms or were lost from their tanks. Control fish from the colony were selected without regard to gross appearance. Incidence rates are based on the number of fish examined histologically (i.e., excluding lost fish). (A) Lines prone to developing zMPNSTs; (B) Lines with no zMPNSTs observed; (C) Various control lines (heterozygous carriers of non-rp mutations) from the colony. NA = not applicable. ND = not determined.
| Gene | Line | Age Range (in months) | Initial Number of Fish | Number Lost | Number of Fish Examined | Number of Grossly-Apparent Tumors | Number of Tumor-Bearing Fish | Number of zMPNSTs | % of Fish Examined with zMPNSTs | |
|---|---|---|---|---|---|---|---|---|---|---|
| A | rpL35 | hi258 | up to 21.5 | 13 | 7 | 6 | 5 | 6 | 6 | 100% |
| rpL14 | hi823 | up to 24.5 | 26 | 4 | 22 | 20 | 21 | 16 | 73% | |
| rpS15a | hi2649 | up to 18 | 9 | 2 | 7 | 4 | 6 | 5 | 71% | |
| rpL36 | hi1807 | up to 23 | 62 | 11 | 51 | 34 | 40 | 33 | 65% | |
| rpS3a | hi1290 | up to 23.5 | 41 | 10 | 31 | 24 | 25 | 20 | 65% | |
| rpL36a | hi10 | up to 22 | 14 | 6 | 8 | 4 | 5 | 5 | 63% | |
| rpS8 | hi1974 | up to 22 | 19 | 6 | 13 | 6 | 9 | 8 | 62% | |
| rpS11 | hi2799 | up to 23.5 | 23 | 8 | 15 | 6 | 9 | 7 | 47% | |
| rpL7 | hi1061 | up to 22.5 | 28 | 6 | 22 | 11 | 11 | 10 | 45% | |
| rpS5 | hi577B | up to 23 | 27 | 4 | 23 | 7 | 12 | 10 | 43% | |
| rpS7 | hi1034B | up to 22 | 19 | 4 | 15 | 3 | 6 | 5 | 33% | |
| rpS28 | hi3883 | up to 23 | 25 | 7 | 18 | 6 | 6 | 6 | 33% | |
| rpL13 | hi1016 | up to 23 | 15 | 4 | 11 | 2 | 5 | 3 | 27% | |
| rpS29 | hi2903 | up to 22 | 18 | 3 | 15 | 4 | 4 | 4 | 27% | |
| rpS18 | hi1026 | up to 24 | 59 | 13 | 46 | 9 | 12 | 8 | 17% | |
| rpL23a | hi2582 | up to 22.5 | 71 | 8 | 63 | 5 | 7 | 6 | 10% | |
| rpL19 | hi1987 | up to 23 | 55 | 16 | 39 | 2 | 4 | 3 | 8% | |
| B | rpL12 | hi3076 | up to 23.5 | 24 | 2 | 22 | 2 | 3 | 0 | 0% |
| rpS12 | hi1227 | all at 22 | 14 | 1 | 13 | 0 | 1 | 0 | 0% | |
| rpL9 | hi1422 | all at 22.5 | 23 | 8 | 15 | 0 | 1 | 0 | 0% | |
| rpL3 | hi2437 | all at 23 | 19 | 2 | 17 | 0 | 1 | 0 | 0% | |
| rpLP1 | hi1444 | up to 22.5 | 18 | 0 | 18 | 1 | 1 | 0 | 0% | |
| rpL6 | hi3655B | up to 24.5 | 42 | 1 | 41 | 0 | 2 | 0 | 0% | |
| rpSa | hi1479 | up to 24.5 | 29 | 7 | 22 | 0 | 1 | 0 | 0% | |
| rpL28 | hi3893 | up to 24.5 | 34 | 8 | 26 | 0 | 1 | 0 | 0% | |
| rpL11 | hi3820B | up to 23.5 | 29 | 2 | 27 | 1 | 1 | 0 | 0% | |
| rpL24 | hi1284 | all at 26 | 18 | 0 | 18 | 0 | 0 | 0 | 0% | |
| rpS15 | hi2430 | all at 23 | 16 | 0 | 16 | 0 | 0 | 0 | 0% | |
| C | NA | Controls | 20 to 26 | ND | ND | 9988 | 167 | 473 | 5 | 0.05% |