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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Mutat Res. 2008 Oct 17;660(1-2):1–11. doi: 10.1016/j.mrfmmm.2008.09.017

Fig. 4. Functional analysis of missense variants in BRCA1.

Fig. 4

A. Quantitative assay in yeast (white bars) and mammalian cells (black bars) performed as described in Fig. 3. The thresholds of activity (see Material and Methods) used for classification are indicated by dotted lines. B. Protein levels determined by western blot using α-LexA DBD polyclonal antibody for yeast extracts (top panels) or mouse α-GAL4 DBD monoclonal antibody in mammalian cell extracts (bottom panel).